rat anti-d1 dopamine receptor (drd1) Search Results


polyclonal rabbit anti d1  (Alomone Labs)
94
Alomone Labs polyclonal rabbit anti d1
Polyclonal Rabbit Anti D1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyclonal rabbit anti d1/product/Alomone Labs
Average 94 stars, based on 1 article reviews
polyclonal rabbit anti d1 - by Bioz Stars, 2026-02
94/100 stars
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anti d1 dopamine receptor  (OriGene)
90
OriGene anti d1 dopamine receptor
RNA sequencing data analysis in nax ( n = 5) and wild–type (wt) ( n = 6) mice. Data analysis shows dopamine receptor <t>D1</t> ( <t>Drd1,</t> A ), Drd3 ( C ), and Drd4 ( D ) are significantly increased in the nax cerebellum. Although an increase in Drd2 ( B ) and Drd5 ( E ) is apparent, statistical significance was not reached. The data in the bar graph are presented as the mean ± SEM, and statistical analysis was performed using an unpaired t –test (* p < 0.05 and ** p < 0.01). RPKM: Reads Per Kilobase of transcript per Million mapped reads.
Anti D1 Dopamine Receptor, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti d1 dopamine receptor/product/OriGene
Average 90 stars, based on 1 article reviews
anti d1 dopamine receptor - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
anti d4 dopamine receptor  (OriGene)
90
OriGene anti d4 dopamine receptor
RNA sequencing data analysis in nax ( n = 5) and wild–type (wt) ( n = 6) mice. Data analysis shows dopamine receptor D1 ( Drd1, A ), Drd3 ( C ), and <t>Drd4</t> ( D ) are significantly increased in the nax cerebellum. Although an increase in Drd2 ( B ) and Drd5 ( E ) is apparent, statistical significance was not reached. The data in the bar graph are presented as the mean ± SEM, and statistical analysis was performed using an unpaired t –test (* p < 0.05 and ** p < 0.01). RPKM: Reads Per Kilobase of transcript per Million mapped reads.
Anti D4 Dopamine Receptor, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti d4 dopamine receptor/product/OriGene
Average 90 stars, based on 1 article reviews
anti d4 dopamine receptor - by Bioz Stars, 2026-02
90/100 stars
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anti d2 dopamine receptor  (OriGene)
90
OriGene anti d2 dopamine receptor
RNA sequencing data analysis in nax ( n = 5) and wild–type (wt) ( n = 6) mice. Data analysis shows dopamine receptor D1 ( Drd1, A ), Drd3 ( C ), and Drd4 ( D ) are significantly increased in the nax cerebellum. Although an increase in <t>Drd2</t> ( B ) and Drd5 ( E ) is apparent, statistical significance was not reached. The data in the bar graph are presented as the mean ± SEM, and statistical analysis was performed using an unpaired t –test (* p < 0.05 and ** p < 0.01). RPKM: Reads Per Kilobase of transcript per Million mapped reads.
Anti D2 Dopamine Receptor, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti d2 dopamine receptor/product/OriGene
Average 90 stars, based on 1 article reviews
anti d2 dopamine receptor - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
anti d5 dopamine receptor  (OriGene)
90
OriGene anti d5 dopamine receptor
RNA sequencing data analysis in nax ( n = 5) and wild–type (wt) ( n = 6) mice. Data analysis shows dopamine receptor D1 ( Drd1, A ), Drd3 ( C ), and Drd4 ( D ) are significantly increased in the nax cerebellum. Although an increase in Drd2 ( B ) and <t>Drd5</t> ( E ) is apparent, statistical significance was not reached. The data in the bar graph are presented as the mean ± SEM, and statistical analysis was performed using an unpaired t –test (* p < 0.05 and ** p < 0.01). RPKM: Reads Per Kilobase of transcript per Million mapped reads.
Anti D5 Dopamine Receptor, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti d5 dopamine receptor/product/OriGene
Average 90 stars, based on 1 article reviews
anti d5 dopamine receptor - by Bioz Stars, 2026-02
90/100 stars
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rat anti-d1 dopamine receptor (drd1)  (Millipore)
90
Millipore rat anti-d1 dopamine receptor (drd1)
RNA sequencing data analysis in nax ( n = 5) and wild–type (wt) ( n = 6) mice. Data analysis shows dopamine receptor D1 ( Drd1, A ), Drd3 ( C ), and Drd4 ( D ) are significantly increased in the nax cerebellum. Although an increase in Drd2 ( B ) and <t>Drd5</t> ( E ) is apparent, statistical significance was not reached. The data in the bar graph are presented as the mean ± SEM, and statistical analysis was performed using an unpaired t –test (* p < 0.05 and ** p < 0.01). RPKM: Reads Per Kilobase of transcript per Million mapped reads.
Rat Anti D1 Dopamine Receptor (Drd1), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rat anti-d1 dopamine receptor (drd1)/product/Millipore
Average 90 stars, based on 1 article reviews
rat anti-d1 dopamine receptor (drd1) - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


RNA sequencing data analysis in nax ( n = 5) and wild–type (wt) ( n = 6) mice. Data analysis shows dopamine receptor D1 ( Drd1, A ), Drd3 ( C ), and Drd4 ( D ) are significantly increased in the nax cerebellum. Although an increase in Drd2 ( B ) and Drd5 ( E ) is apparent, statistical significance was not reached. The data in the bar graph are presented as the mean ± SEM, and statistical analysis was performed using an unpaired t –test (* p < 0.05 and ** p < 0.01). RPKM: Reads Per Kilobase of transcript per Million mapped reads.

Journal: International Journal of Molecular Sciences

Article Title: Alteration of the Dopamine Receptors’ Expression in the Cerebellum of the Lysosomal Acid Phosphatase 2 Mutant (Naked–Ataxia ( NAX )) Mouse

doi: 10.3390/ijms21082914

Figure Lengend Snippet: RNA sequencing data analysis in nax ( n = 5) and wild–type (wt) ( n = 6) mice. Data analysis shows dopamine receptor D1 ( Drd1, A ), Drd3 ( C ), and Drd4 ( D ) are significantly increased in the nax cerebellum. Although an increase in Drd2 ( B ) and Drd5 ( E ) is apparent, statistical significance was not reached. The data in the bar graph are presented as the mean ± SEM, and statistical analysis was performed using an unpaired t –test (* p < 0.05 and ** p < 0.01). RPKM: Reads Per Kilobase of transcript per Million mapped reads.

Article Snippet: D1: rabbit polyclonal anti–D1 dopamine receptor (TA328798, anti–Drd1, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA); D2: rabbit polyclonal anti–D2 dopamine receptor (TA328800, anti–Drd2, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA), produced against recombinant rat dopamine receptor 2 (DR2); D3: rabbit polyclonal anti–D3 dopamine receptor (TA328800, anti–Drd3, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA), produced against recombinant rat dopamine receptor 3 (DR3); D4: rabbit polyclonal anti–D4 dopamine receptor (TA321202, anti–DRD4, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA), produced against recombinant rat dopamine receptor D4 (DRD4); and D5: rabbit polyclonal anti–D5 dopamine receptor (TA328802, anti–Drd5, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA), produced against recombinant rat dopamine receptor 5 (DR5).

Techniques: RNA Sequencing Assay

Frontal sections of wt and nax mouse cerebella: DRD1 expression at P5 and P17. ( A ) Immunoperoxidase staining for DRD1 in the wt cerebellum on P5 shows the immunoreactivity in the Purkinje cell layer (Pcl) and white matter (wm) but not in the external germinal zone (egz) and granular layer (gl). ( B ) Immunoperoxidase staining for DRD1 in the nax cerebellum shows similar immunoreactivity as in the wt littermates at P5. ( C ) DRD1 immunostaining of the frontal sections of the wt cerebellum at P17 shows immunoreactivity in the Purkinje cell (PC) somata, the Pcl, and dendrites in the molecular layer (ml) and in the gl but not in the wm. ( D ) DRD1 immunostaining of the frontal sections of the nax cerebellum on P17 shows immunoreactivity in the PCs in the ml/Pcl. ( E ) Western blot analysis of whole cerebellar lysates revealed no significant differences in DRD1 expression between wt and nax cerebella at P5 and P17 (wt: n = 3 and nax : n = 3). The data in the bar graphs are presented as the means of three independent experiments ± SEM, and statistical analysis was performed using a two–way ANOVA. P; postnatal. Scale bars: 100 μm in A, B, C, and D.

Journal: International Journal of Molecular Sciences

Article Title: Alteration of the Dopamine Receptors’ Expression in the Cerebellum of the Lysosomal Acid Phosphatase 2 Mutant (Naked–Ataxia ( NAX )) Mouse

doi: 10.3390/ijms21082914

Figure Lengend Snippet: Frontal sections of wt and nax mouse cerebella: DRD1 expression at P5 and P17. ( A ) Immunoperoxidase staining for DRD1 in the wt cerebellum on P5 shows the immunoreactivity in the Purkinje cell layer (Pcl) and white matter (wm) but not in the external germinal zone (egz) and granular layer (gl). ( B ) Immunoperoxidase staining for DRD1 in the nax cerebellum shows similar immunoreactivity as in the wt littermates at P5. ( C ) DRD1 immunostaining of the frontal sections of the wt cerebellum at P17 shows immunoreactivity in the Purkinje cell (PC) somata, the Pcl, and dendrites in the molecular layer (ml) and in the gl but not in the wm. ( D ) DRD1 immunostaining of the frontal sections of the nax cerebellum on P17 shows immunoreactivity in the PCs in the ml/Pcl. ( E ) Western blot analysis of whole cerebellar lysates revealed no significant differences in DRD1 expression between wt and nax cerebella at P5 and P17 (wt: n = 3 and nax : n = 3). The data in the bar graphs are presented as the means of three independent experiments ± SEM, and statistical analysis was performed using a two–way ANOVA. P; postnatal. Scale bars: 100 μm in A, B, C, and D.

Article Snippet: D1: rabbit polyclonal anti–D1 dopamine receptor (TA328798, anti–Drd1, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA); D2: rabbit polyclonal anti–D2 dopamine receptor (TA328800, anti–Drd2, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA), produced against recombinant rat dopamine receptor 2 (DR2); D3: rabbit polyclonal anti–D3 dopamine receptor (TA328800, anti–Drd3, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA), produced against recombinant rat dopamine receptor 3 (DR3); D4: rabbit polyclonal anti–D4 dopamine receptor (TA321202, anti–DRD4, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA), produced against recombinant rat dopamine receptor D4 (DRD4); and D5: rabbit polyclonal anti–D5 dopamine receptor (TA328802, anti–Drd5, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA), produced against recombinant rat dopamine receptor 5 (DR5).

Techniques: Expressing, Immunoperoxidase Staining, Immunostaining, Western Blot

RNA sequencing data analysis in nax ( n = 5) and wild–type (wt) ( n = 6) mice. Data analysis shows dopamine receptor D1 ( Drd1, A ), Drd3 ( C ), and Drd4 ( D ) are significantly increased in the nax cerebellum. Although an increase in Drd2 ( B ) and Drd5 ( E ) is apparent, statistical significance was not reached. The data in the bar graph are presented as the mean ± SEM, and statistical analysis was performed using an unpaired t –test (* p < 0.05 and ** p < 0.01). RPKM: Reads Per Kilobase of transcript per Million mapped reads.

Journal: International Journal of Molecular Sciences

Article Title: Alteration of the Dopamine Receptors’ Expression in the Cerebellum of the Lysosomal Acid Phosphatase 2 Mutant (Naked–Ataxia ( NAX )) Mouse

doi: 10.3390/ijms21082914

Figure Lengend Snippet: RNA sequencing data analysis in nax ( n = 5) and wild–type (wt) ( n = 6) mice. Data analysis shows dopamine receptor D1 ( Drd1, A ), Drd3 ( C ), and Drd4 ( D ) are significantly increased in the nax cerebellum. Although an increase in Drd2 ( B ) and Drd5 ( E ) is apparent, statistical significance was not reached. The data in the bar graph are presented as the mean ± SEM, and statistical analysis was performed using an unpaired t –test (* p < 0.05 and ** p < 0.01). RPKM: Reads Per Kilobase of transcript per Million mapped reads.

Article Snippet: D1: rabbit polyclonal anti–D1 dopamine receptor (TA328798, anti–Drd1, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA); D2: rabbit polyclonal anti–D2 dopamine receptor (TA328800, anti–Drd2, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA), produced against recombinant rat dopamine receptor 2 (DR2); D3: rabbit polyclonal anti–D3 dopamine receptor (TA328800, anti–Drd3, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA), produced against recombinant rat dopamine receptor 3 (DR3); D4: rabbit polyclonal anti–D4 dopamine receptor (TA321202, anti–DRD4, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA), produced against recombinant rat dopamine receptor D4 (DRD4); and D5: rabbit polyclonal anti–D5 dopamine receptor (TA328802, anti–Drd5, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA), produced against recombinant rat dopamine receptor 5 (DR5).

Techniques: RNA Sequencing Assay

Frontal and sagittal sections of wt and nax mouse cerebella: DRD4 expression at P5 and P17. ( A , B ) Immunoperoxidase staining for DRD4 in wt ( A ) and nax ( B ) cerebella at P5 shows immunoreactivity in the external germinal zone (egz) and no or only weak immunoreactivity in the Pcl. ( C – G ) Sagittal section of P17 wt cerebellum immunostained for DRD4 reveals strong immunoreactivity within the cerebellar cortex. In the anterior zone, the expression of DRD4 is present in PC dendrites in the molecular layer (ml), while PC somata and dendrites exhibit immunoreactivity in the nodular zone ( C ). Frontal section of P17 wt cerebellum immunostained with DRD4 shows strong immunoreactivity in a subset of PCs (indicated by black arrowheads), while another subset of PCs displays no (white arrowhead) or only weak expression (arrow) in the cerebellar cortex ( D ). ( E – G ) Immunoperoxidase staining for DRD4 in the wt cerebellum at P17 shows a striped expression pattern in lobule IXd ( E , F ) and more uniform expression in PCs in lobule X ( G ). ( H – J ) DRD4 immunostaining of a frontal section of the nax cerebellum at P17 shows immunoreactivity in a subset of PCs (asterisks) that follows a similar stripes pattern in lobule III ( H ) and IX ( I ) but is uniform in lobule X ( J ). ( K ) Western blot analysis of DRD4 expression in wt and nax cerebellum at P5 and P17 shows an apparent increase in expression in nax cerebella at both time–points (wt: n = 3 and nax : n = 3); however, these differences did not reach statistical significance. The data in the bar graphs are presented as the means of three independent experiments ± SEM, and statistical analysis was performed using two–way ANOVA. P; postnatal. Scale bars: 100 μm in A; 50 μm in B; 500 μm in C; 20 μm in D; 200 μm in E, F, G, H, and I; and 500 μm in J.

Journal: International Journal of Molecular Sciences

Article Title: Alteration of the Dopamine Receptors’ Expression in the Cerebellum of the Lysosomal Acid Phosphatase 2 Mutant (Naked–Ataxia ( NAX )) Mouse

doi: 10.3390/ijms21082914

Figure Lengend Snippet: Frontal and sagittal sections of wt and nax mouse cerebella: DRD4 expression at P5 and P17. ( A , B ) Immunoperoxidase staining for DRD4 in wt ( A ) and nax ( B ) cerebella at P5 shows immunoreactivity in the external germinal zone (egz) and no or only weak immunoreactivity in the Pcl. ( C – G ) Sagittal section of P17 wt cerebellum immunostained for DRD4 reveals strong immunoreactivity within the cerebellar cortex. In the anterior zone, the expression of DRD4 is present in PC dendrites in the molecular layer (ml), while PC somata and dendrites exhibit immunoreactivity in the nodular zone ( C ). Frontal section of P17 wt cerebellum immunostained with DRD4 shows strong immunoreactivity in a subset of PCs (indicated by black arrowheads), while another subset of PCs displays no (white arrowhead) or only weak expression (arrow) in the cerebellar cortex ( D ). ( E – G ) Immunoperoxidase staining for DRD4 in the wt cerebellum at P17 shows a striped expression pattern in lobule IXd ( E , F ) and more uniform expression in PCs in lobule X ( G ). ( H – J ) DRD4 immunostaining of a frontal section of the nax cerebellum at P17 shows immunoreactivity in a subset of PCs (asterisks) that follows a similar stripes pattern in lobule III ( H ) and IX ( I ) but is uniform in lobule X ( J ). ( K ) Western blot analysis of DRD4 expression in wt and nax cerebellum at P5 and P17 shows an apparent increase in expression in nax cerebella at both time–points (wt: n = 3 and nax : n = 3); however, these differences did not reach statistical significance. The data in the bar graphs are presented as the means of three independent experiments ± SEM, and statistical analysis was performed using two–way ANOVA. P; postnatal. Scale bars: 100 μm in A; 50 μm in B; 500 μm in C; 20 μm in D; 200 μm in E, F, G, H, and I; and 500 μm in J.

Article Snippet: D1: rabbit polyclonal anti–D1 dopamine receptor (TA328798, anti–Drd1, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA); D2: rabbit polyclonal anti–D2 dopamine receptor (TA328800, anti–Drd2, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA), produced against recombinant rat dopamine receptor 2 (DR2); D3: rabbit polyclonal anti–D3 dopamine receptor (TA328800, anti–Drd3, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA), produced against recombinant rat dopamine receptor 3 (DR3); D4: rabbit polyclonal anti–D4 dopamine receptor (TA321202, anti–DRD4, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA), produced against recombinant rat dopamine receptor D4 (DRD4); and D5: rabbit polyclonal anti–D5 dopamine receptor (TA328802, anti–Drd5, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA), produced against recombinant rat dopamine receptor 5 (DR5).

Techniques: Expressing, Immunoperoxidase Staining, Immunostaining, Western Blot

RNA sequencing data analysis in nax ( n = 5) and wild–type (wt) ( n = 6) mice. Data analysis shows dopamine receptor D1 ( Drd1, A ), Drd3 ( C ), and Drd4 ( D ) are significantly increased in the nax cerebellum. Although an increase in Drd2 ( B ) and Drd5 ( E ) is apparent, statistical significance was not reached. The data in the bar graph are presented as the mean ± SEM, and statistical analysis was performed using an unpaired t –test (* p < 0.05 and ** p < 0.01). RPKM: Reads Per Kilobase of transcript per Million mapped reads.

Journal: International Journal of Molecular Sciences

Article Title: Alteration of the Dopamine Receptors’ Expression in the Cerebellum of the Lysosomal Acid Phosphatase 2 Mutant (Naked–Ataxia ( NAX )) Mouse

doi: 10.3390/ijms21082914

Figure Lengend Snippet: RNA sequencing data analysis in nax ( n = 5) and wild–type (wt) ( n = 6) mice. Data analysis shows dopamine receptor D1 ( Drd1, A ), Drd3 ( C ), and Drd4 ( D ) are significantly increased in the nax cerebellum. Although an increase in Drd2 ( B ) and Drd5 ( E ) is apparent, statistical significance was not reached. The data in the bar graph are presented as the mean ± SEM, and statistical analysis was performed using an unpaired t –test (* p < 0.05 and ** p < 0.01). RPKM: Reads Per Kilobase of transcript per Million mapped reads.

Article Snippet: D1: rabbit polyclonal anti–D1 dopamine receptor (TA328798, anti–Drd1, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA); D2: rabbit polyclonal anti–D2 dopamine receptor (TA328800, anti–Drd2, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA), produced against recombinant rat dopamine receptor 2 (DR2); D3: rabbit polyclonal anti–D3 dopamine receptor (TA328800, anti–Drd3, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA), produced against recombinant rat dopamine receptor 3 (DR3); D4: rabbit polyclonal anti–D4 dopamine receptor (TA321202, anti–DRD4, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA), produced against recombinant rat dopamine receptor D4 (DRD4); and D5: rabbit polyclonal anti–D5 dopamine receptor (TA328802, anti–Drd5, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA), produced against recombinant rat dopamine receptor 5 (DR5).

Techniques: RNA Sequencing Assay

Frontal sections of wt and nax mouse cerebella: DRD2 expression at P5 and P17. Immunoperoxidase staining for DRD2 in wt ( A ) and nax ( B ) cerebella at P5 shows weak immunoreactivity in the Pcl of wt but relatively strong immunoreactivity in PC somata of the nax cerebellum. ( C , D ) Immunoperoxidase staining for DRD2 in wt ( C ) and nax ( D ) cerebella at P17 shows weak immunoreactivity in PCs, with no differences between the two groups. ( E ) Western blot analysis of whole cerebellar lysates revealed no significant differences in DRD2 protein expression between wt and nax cerebella at P5 and P17 (wt: n = 3 and nax : n = 3). The data in the bar graphs are presented as the means of three independent experiments ± SEM; statistical analysis was performed using two–way ANOVA. P; postnatal. Scale bars: 100 μm in A, C, and D and 50 μm in B.

Journal: International Journal of Molecular Sciences

Article Title: Alteration of the Dopamine Receptors’ Expression in the Cerebellum of the Lysosomal Acid Phosphatase 2 Mutant (Naked–Ataxia ( NAX )) Mouse

doi: 10.3390/ijms21082914

Figure Lengend Snippet: Frontal sections of wt and nax mouse cerebella: DRD2 expression at P5 and P17. Immunoperoxidase staining for DRD2 in wt ( A ) and nax ( B ) cerebella at P5 shows weak immunoreactivity in the Pcl of wt but relatively strong immunoreactivity in PC somata of the nax cerebellum. ( C , D ) Immunoperoxidase staining for DRD2 in wt ( C ) and nax ( D ) cerebella at P17 shows weak immunoreactivity in PCs, with no differences between the two groups. ( E ) Western blot analysis of whole cerebellar lysates revealed no significant differences in DRD2 protein expression between wt and nax cerebella at P5 and P17 (wt: n = 3 and nax : n = 3). The data in the bar graphs are presented as the means of three independent experiments ± SEM; statistical analysis was performed using two–way ANOVA. P; postnatal. Scale bars: 100 μm in A, C, and D and 50 μm in B.

Article Snippet: D1: rabbit polyclonal anti–D1 dopamine receptor (TA328798, anti–Drd1, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA); D2: rabbit polyclonal anti–D2 dopamine receptor (TA328800, anti–Drd2, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA), produced against recombinant rat dopamine receptor 2 (DR2); D3: rabbit polyclonal anti–D3 dopamine receptor (TA328800, anti–Drd3, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA), produced against recombinant rat dopamine receptor 3 (DR3); D4: rabbit polyclonal anti–D4 dopamine receptor (TA321202, anti–DRD4, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA), produced against recombinant rat dopamine receptor D4 (DRD4); and D5: rabbit polyclonal anti–D5 dopamine receptor (TA328802, anti–Drd5, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA), produced against recombinant rat dopamine receptor 5 (DR5).

Techniques: Expressing, Immunoperoxidase Staining, Western Blot

RNA sequencing data analysis in nax ( n = 5) and wild–type (wt) ( n = 6) mice. Data analysis shows dopamine receptor D1 ( Drd1, A ), Drd3 ( C ), and Drd4 ( D ) are significantly increased in the nax cerebellum. Although an increase in Drd2 ( B ) and Drd5 ( E ) is apparent, statistical significance was not reached. The data in the bar graph are presented as the mean ± SEM, and statistical analysis was performed using an unpaired t –test (* p < 0.05 and ** p < 0.01). RPKM: Reads Per Kilobase of transcript per Million mapped reads.

Journal: International Journal of Molecular Sciences

Article Title: Alteration of the Dopamine Receptors’ Expression in the Cerebellum of the Lysosomal Acid Phosphatase 2 Mutant (Naked–Ataxia ( NAX )) Mouse

doi: 10.3390/ijms21082914

Figure Lengend Snippet: RNA sequencing data analysis in nax ( n = 5) and wild–type (wt) ( n = 6) mice. Data analysis shows dopamine receptor D1 ( Drd1, A ), Drd3 ( C ), and Drd4 ( D ) are significantly increased in the nax cerebellum. Although an increase in Drd2 ( B ) and Drd5 ( E ) is apparent, statistical significance was not reached. The data in the bar graph are presented as the mean ± SEM, and statistical analysis was performed using an unpaired t –test (* p < 0.05 and ** p < 0.01). RPKM: Reads Per Kilobase of transcript per Million mapped reads.

Article Snippet: D1: rabbit polyclonal anti–D1 dopamine receptor (TA328798, anti–Drd1, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA); D2: rabbit polyclonal anti–D2 dopamine receptor (TA328800, anti–Drd2, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA), produced against recombinant rat dopamine receptor 2 (DR2); D3: rabbit polyclonal anti–D3 dopamine receptor (TA328800, anti–Drd3, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA), produced against recombinant rat dopamine receptor 3 (DR3); D4: rabbit polyclonal anti–D4 dopamine receptor (TA321202, anti–DRD4, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA), produced against recombinant rat dopamine receptor D4 (DRD4); and D5: rabbit polyclonal anti–D5 dopamine receptor (TA328802, anti–Drd5, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA), produced against recombinant rat dopamine receptor 5 (DR5).

Techniques: RNA Sequencing Assay

Sagittal sections of wt and nax mouse cerebella: DRD5 expression at P5 and P17. ( A , B ) Immunoperoxidase staining for DRD5 in wt ( A ) and nax ( B ) cerebella at P5 shows weak immunoreactivity in the Pcl. ( C , D ) Immunoperoxidase staining for DRD5 at P17 shows weak immunoreactivity in PCs in the wt ( C ) and nax ( D ) cerebellar cortex. ( E ) Western blot analysis of whole cerebellar lysates revealed no significant differences in DRD5 protein expression between wt and nax cerebella at P5 and P17 (wt: n = 3 and nax : n = 3). The data in the bar graphs are presented as the means of three independent experiments ± SEM, and statistical analysis was performed using two–way ANOVA. P; postnatal. Scale bars: 50 μm in A and B and 100 μm in C and D.

Journal: International Journal of Molecular Sciences

Article Title: Alteration of the Dopamine Receptors’ Expression in the Cerebellum of the Lysosomal Acid Phosphatase 2 Mutant (Naked–Ataxia ( NAX )) Mouse

doi: 10.3390/ijms21082914

Figure Lengend Snippet: Sagittal sections of wt and nax mouse cerebella: DRD5 expression at P5 and P17. ( A , B ) Immunoperoxidase staining for DRD5 in wt ( A ) and nax ( B ) cerebella at P5 shows weak immunoreactivity in the Pcl. ( C , D ) Immunoperoxidase staining for DRD5 at P17 shows weak immunoreactivity in PCs in the wt ( C ) and nax ( D ) cerebellar cortex. ( E ) Western blot analysis of whole cerebellar lysates revealed no significant differences in DRD5 protein expression between wt and nax cerebella at P5 and P17 (wt: n = 3 and nax : n = 3). The data in the bar graphs are presented as the means of three independent experiments ± SEM, and statistical analysis was performed using two–way ANOVA. P; postnatal. Scale bars: 50 μm in A and B and 100 μm in C and D.

Article Snippet: D1: rabbit polyclonal anti–D1 dopamine receptor (TA328798, anti–Drd1, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA); D2: rabbit polyclonal anti–D2 dopamine receptor (TA328800, anti–Drd2, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA), produced against recombinant rat dopamine receptor 2 (DR2); D3: rabbit polyclonal anti–D3 dopamine receptor (TA328800, anti–Drd3, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA), produced against recombinant rat dopamine receptor 3 (DR3); D4: rabbit polyclonal anti–D4 dopamine receptor (TA321202, anti–DRD4, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA), produced against recombinant rat dopamine receptor D4 (DRD4); and D5: rabbit polyclonal anti–D5 dopamine receptor (TA328802, anti–Drd5, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA), produced against recombinant rat dopamine receptor 5 (DR5).

Techniques: Expressing, Immunoperoxidase Staining, Western Blot